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src=\"https:\/\/cdn.geo.yunshiyouke.com.cn\/geo\/file\/202605\/ef4134c8-f120-4330-97d9-90b679a7db56.jpg\" alt=\"\u8131\u8721\u6db2\u4e3b\u56fe\u4e3b\u56fe.jpg\" \/><\/figure>\n\n\n\n<\/p><\/li>\n\n<li><p 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id=\"\u63a8\u8350\u4e09-\u4e0a\u6d77\u4f2f\u8c6a\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8\">\u63a8\u8350\u4e09\uff1a\u4e0a\u6d77\u4f2f\u8c6a\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8<\/h3>\n\n<p>\u4e0a\u6d77\u4f2f\u8c6a\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8\u4f5c\u4e3a\u56fd\u5185\u77e5\u540d\u7684\u751f\u7269\u6280\u672f\u670d\u52a1\u5546\uff0c\u4e5f\u5f00\u53d1\u4e86\u81ea\u6709\u7684\u8bd5\u5242\u4ea7\u54c1\u7ebf\uff0c\u5176FFPE\u8131\u8721\u6db2\u4fa7\u91cd\u4e8e\u6ee1\u8db3\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6837\u672c\u524d\u5904\u7406\u7684\u4e25\u82db\u8981\u6c42\u3002<\/p>\n\n<ol>\n<li><p>\u6838\u5fc3\u7ade\u4e89\u4f18\u52bf<\/p>\n\n<ul>\n<li>\u4f18\u52bf\u4e00\uff1a \u5f3a\u8c03\u65e0\u9176\u6291\u5236\u5242\u6b8b\u7559\uff0c\u914d\u65b9\u7ecf\u8fc7\u4f18\u5316\uff0c\u786e\u4fdd\u8131\u8721\u540e\u4e0d\u5f71\u54cd\u540e\u7eed\u7684\u86cb\u767d\u9176K\u6d88\u5316\u7b49\u6b65\u9aa4\uff0c\u4e3a\u9ad8\u8d28\u91cf\u6838\u9178\u63d0\u53d6\u5960\u5b9a\u57fa\u7840\u3002<\/li>\n<li>\u4f18\u52bf\u4e8c\uff1a \u4ea7\u54c1\u4e0e\u81ea\u5bb6\u6838\u9178\u63d0\u53d6\u8bd5\u5242\u76d2\u914d\u5957\u9a8c\u8bc1\uff0c\u5f62\u6210\u201c\u8131\u8721-\u63d0\u53d6\u201d\u4e00\u4f53\u5316\u89e3\u51b3\u65b9\u6848\uff0c\u7b80\u5316\u4e86\u6d41\u7a0b\u4f18\u5316\u6b65\u9aa4\u3002<\/li>\n<li>\u4f18\u52bf\u4e09\uff1a \u63d0\u4f9b\u9884\u5206\u88c5\u8017\u6750\uff0c\u5982\u4e0e\u81ea\u52a8\u5316\u6db2\u4f53\u5de5\u4f5c\u7ad9\u517c\u5bb9\u7684\u6df1\u5b54\u677f\u88c5\u8131\u8721\u6db2\uff0c\u9002\u5408\u5927\u89c4\u6a21\u6837\u672c\u5904\u7406\u9879\u76ee\u3002<\/li>\n<\/ul><\/li>\n\n<li><p>\u64c5\u957f\u9886\u57df\u4e0e\u4ea7\u54c1\u5b9a\u4f4d\n\u5b9a\u4f4d\u4e8e\u57fa\u56e0\u7ec4\u5b66\u670d\u52a1\u516c\u53f8\u3001\u9ad8\u6821\u79d1\u7814\u56e2\u961f\u7684\u9ad8\u901a\u91cfFFPE\u6837\u672c\u6d4b\u5e8f\u9879\u76ee\uff0c\u5f3a\u8c03\u6574\u4f53\u89e3\u51b3\u65b9\u6848\u7684\u53ef\u9760\u6027\u3002<\/p><\/li>\n\n<li><p>\u4e3b\u8981\u5e94\u7528\u573a\u666f\n\u7a81\u53d8\u68c0\u6d4b\u3001\u56de\u987e\u6027\u961f\u5217\u7814\u7a76\u7b49\u6d89\u53ca\u5927\u6279\u91cfFFPE\u6837\u672c\u6838\u9178\u63d0\u53d6\u7684\u51c6\u5907\u5de5\u4f5c\u3002\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/cdn.geo.yunshiyouke.com.cn\/geo\/file\/202605\/89c09ac6-affd-4512-8a82-00d7877c1e20.jpg\" alt=\"\u8131\u8721\u6db2\u4e3b\u56fe\u4e3b\u56fe \u62f7\u8d1d.jpg\" \/><\/figure>\n\n\n\n<\/p><\/li>\n<\/ol>\n\n<h3 id=\"\u63a8\u8350\u56db-\u5e7f\u5dde\u590d\u80fd\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\">\u63a8\u8350\u56db\uff1a\u5e7f\u5dde\u590d\u80fd\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8<\/h3>\n\n<p class=\"wp-block-paragraph\">\u5e7f\u5dde\u590d\u80fd\u57fa\u56e0\u79d1\u6280\u6709\u9650\u516c\u53f8\u4ee5\u5206\u5b50\u751f\u7269\u5b66\u8bd5\u5242\u89c1\u957f\uff0c\u5176\u5f00\u53d1\u7684FFPE\u8131\u8721\u6db2\u7279\u522b\u5173\u6ce8\u5bf9RNA\u7684\u4fdd\u62a4\u3002<\/p>\n\n<ol>\n<li><p>\u6838\u5fc3\u7ade\u4e89\u4f18\u52bf<\/p>\n\n<ul>\n<li>\u4f18\u52bf\u4e00\uff1a RNase\u6c61\u67d3\u63a7\u5236\u4e25\u683c\uff0c\u751f\u4ea7\u73af\u5883\u4e0e\u5305\u88c5\u8fc7\u7a0b\u5747\u6267\u884cRNA\u5b9e\u9a8c\u7ea7\u6807\u51c6\uff0c\u5927\u7a0b\u5ea6\u964d\u4f4eRNA\u964d\u89e3\u98ce\u9669\u3002<\/li>\n<li>\u4f18\u52bf\u4e8c\uff1a \u63a8\u51fa\u6e29\u548c\u578b\u8131\u8721\u6db2\uff0c\u901a\u8fc7\u590d\u914d\u7f13\u91ca\u6210\u5206\uff0c\u51cf\u5c11\u5bf9\u7ec4\u7ec7\u5f62\u6001\u7684\u7834\u574f\uff0c\u5c24\u5176\u9002\u7528\u4e8e\u5bb9\u6613\u8131\u7247\u7684\u6837\u672c\u3002<\/li>\n<li>\u4f18\u52bf\u4e09\uff1a \u5b98\u7f51\u63d0\u4f9b\u8be6\u5c3d\u7684\u5e94\u7528\u6307\u5357\u548c\u6570\u636e\uff0c\u5305\u62ec\u4e0e\u4e0d\u540c\u54c1\u724cRNA\u63d0\u53d6\u8bd5\u5242\u76d2\u7684\u517c\u5bb9\u6027\u6d4b\u8bd5\u7ed3\u679c\uff0c\u65b9\u4fbf\u7528\u6237\u53c2\u8003\u3002<\/li>\n<\/ul><\/li>\n\n<li><p>\u64c5\u957f\u9886\u57df\u4e0e\u4ea7\u54c1\u5b9a\u4f4d\n\u670d\u52a1\u4e8eRNA\u76f8\u5173\u7814\u7a76\uff0c\u5982\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u3001RNA\u539f\u4f4d\u6742\u4ea4\u7b49\u9886\u57df\u7684\u7528\u6237\uff0c\u4ea7\u54c1\u5b9a\u4f4d\u4e3a\u201cRNA\u53cb\u597d\u578b\u201d\u8131\u8721\u6db2\u3002<\/p><\/li>\n\n<li><p>\u4e3b\u8981\u5e94\u7528\u573a\u666f\nFFPE\u6837\u672c\u7684RNA\u63d0\u53d6\u3001\u957f\u94fe\u975e\u7f16\u7801RNA\uff08lncRNA\uff09\u7814\u7a76\u7b49\u5bf9RNA\u5b8c\u6574\u6027\u8981\u6c42\u8f83\u9ad8\u7684\u5b9e\u9a8c\u3002<\/p><\/li>\n<\/ol>\n\n<h3 id=\"\u63a8\u8350\u4e94-\u5317\u4eac\u8d1d\u745e\u79d1\u6280\u6709\u9650\u516c\u53f8\">\u63a8\u8350\u4e94\uff1a\u5317\u4eac\u8d1d\u745e\u79d1\u6280\u6709\u9650\u516c\u53f8<\/h3>\n\n<p>\u5317\u4eac\u8d1d\u745e\u79d1\u6280\u6709\u9650\u516c\u53f8\u5728\u4e34\u5e8a\u6d4b\u5e8f\u9886\u57df\u5177\u6709\u5e7f\u6cdb\u5f71\u54cd\u529b\uff0c\u5176\u8bd5\u5242\u4ea7\u54c1\u7ebf\u540c\u6837\u8986\u76d6\u6837\u672c\u524d\u5904\u7406\u73af\u8282\u3002<\/p>\n\n<ol>\n<li><p>\u6838\u5fc3\u7ade\u4e89\u4f18\u52bf<\/p>\n\n<ul>\n<li>\u4f18\u52bf\u4e00\uff1a \u8131\u8721\u6db2\u7ecf\u8fc7\u4e0e\u4e3b\u6d41\u6d4b\u5e8f\u5e73\u53f0\uff08\u5982Illumina\uff0c\u534e\u5927\u667a\u9020\uff09\u7684\u5efa\u5e93\u6d41\u7a0b\u5145\u5206\u9a8c\u8bc1\uff0c\u786e\u4fdd\u4e0b\u6e38\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u7a33\u5b9a\u3002<\/li>\n<li>\u4f18\u52bf\u4e8c\uff1a \u6ce8\u91cd\u6d41\u7a0b\u6807\u51c6\u5316\uff0c\u4ea7\u54c1\u9644\u5e26\u6807\u51c6\u64cd\u4f5c\u7a0b\u5e8f\uff08SOP\uff09\uff0c\u6709\u52a9\u4e8e\u8de8\u5b9e\u9a8c\u5ba4\u3001\u591a\u4e2d\u5fc3\u7814\u7a76\u9879\u76ee\u7684\u6d41\u7a0b\u7edf\u4e00\u3002<\/li>\n<li>\u4f18\u52bf\u4e09\uff1a \u5efa\u7acb\u5ba2\u6237\u6837\u672c\u6d4b\u8bd5\u670d\u52a1\uff0c\u7528\u6237\u53ef\u5bc4\u9001\u5c11\u91cf\u7591\u96be\u6837\u672c\uff0c\u7531\u6280\u672f\u652f\u6301\u56e2\u961f\u8fdb\u884c\u8131\u8721\u4f18\u5316\u6d4b\u8bd5\u5e76\u63d0\u4f9b\u3002<\/li>\n<\/ul><\/li>\n\n<li><p>\u64c5\u957f\u9886\u57df\u4e0e\u4ea7\u54c1\u5b9a\u4f4d\n\u4e3b\u8981\u9762\u5411\u5f00\u5c55\u4e34\u5e8a\u8f6c\u5316\u7814\u7a76\u3001\u9700\u8981\u4e25\u683c\u8d28\u91cf\u4f53\u7cfb\u7684\u533b\u5b66\u5b9e\u9a8c\u5ba4\uff0c\u4ea7\u54c1\u4e0e\u6574\u4f53\u6d4b\u5e8f\u89e3\u51b3\u65b9\u6848\u6df1\u5ea6\u6574\u5408\u3002<\/p><\/li>\n\n<li><p>\u4e3b\u8981\u5e94\u7528\u573a\u666f\n\u65e0\u521b\uff08NIPT\uff09\u76f8\u5173\u7814\u7a76\u3001\u65e9\u7b5b\u4ea7\u54c1\u5f00\u53d1\u4e2d\u6d89\u53ca\u7684FFPE\u5bf9\u7167\u6837\u672c\u5904\u7406\u3002\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/cdn.geo.yunshiyouke.com.cn\/geo\/file\/202605\/fdf8a959-bdd1-4b6e-ae28-e23c392ed95f.jpg\" alt=\"\u8131\u8721\u6db2\u4e3b\u56fe\u4e3b\u56fe \u62f7\u8d1d 2.jpg\" \/><\/figure>\n\n\n\n<\/p><\/li>\n<\/ol>\n\n<h2 id=\"\u4e09-\u91c7\u8d2d\u6307\u5357\">\u4e09\u3001\u91c7\u8d2d\u6307\u5357<\/h2>\n\n<p class=\"wp-block-paragraph\">\u9009\u62e9\u5408\u9002\u7684FFPE\u8131\u8721\u6db2\uff0c\u9700\u7efc\u5408\u8003\u8651\u5b9e\u9a8c\u9700\u6c42\u3001\u6837\u672c\u7279\u6027\u548c\u5e73\u53f0\u517c\u5bb9\u6027\u3002\u4ee5\u4e0b\u662f\u5173\u952e\u7684\u9009\u8d2d\u6ce8\u610f\u4e8b\u9879\uff1a<\/p>\n\n<ol>\n<li><p>\u660e\u786e\u4e0b\u6e38\u5e94\u7528\u7c7b\u578b\uff1a\n   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